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perk polyclonal rabbit primary antibody  (OriGene)


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    OriGene perk polyclonal rabbit primary antibody
    Transient transfection of A549 cells with ORF3a-mRNA. ( a ) Evaluation of ORF3a mRNA levels at 48, 72 and 96 h post-transfection by real-time PCR. ORF3a mRNA levels were normalized to GAPDH gene. ( b ) Detection of recombinant ORF3a protein levels 48, 72 and 96 h post-transfection by Western blotting. For the detection of recombinant ORF3a, a primary <t>polyclonal</t> anti-His tag antibody was used. ORF3a protein expression normalized to GAPDH protein levels. ( c ) Detection of Green Fluorescent Protein (GFP) protein levels 48, 72 and 96 h post-transfection by Western blotting. For the detection of GFP, a primary polyclonal anti-GFP antibody was used. GFP protein expression normalized to GAPDH protein levels. The notation (***) indicate statistically significant differences ( p ≤ 0.001) compared to control cells.
    Perk Polyclonal Rabbit Primary Antibody, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/perk+polyclonal+rabbit+primary+antibody/pmc10296123-74-0-8?v=OriGene
    Average 91 stars, based on 1 article reviews
    perk polyclonal rabbit primary antibody - by Bioz Stars, 2026-08
    91/100 stars

    Images

    1) Product Images from "Inhibition of PERK Kinase, an Orchestrator of the Unfolded Protein Response (UPR), Significantly Reduces Apoptosis and Inflammation of Lung Epithelial Cells Triggered by SARS-CoV-2 ORF3a Protein"

    Article Title: Inhibition of PERK Kinase, an Orchestrator of the Unfolded Protein Response (UPR), Significantly Reduces Apoptosis and Inflammation of Lung Epithelial Cells Triggered by SARS-CoV-2 ORF3a Protein

    Journal: Biomedicines

    doi: 10.3390/biomedicines11061585

    Transient transfection of A549 cells with ORF3a-mRNA. ( a ) Evaluation of ORF3a mRNA levels at 48, 72 and 96 h post-transfection by real-time PCR. ORF3a mRNA levels were normalized to GAPDH gene. ( b ) Detection of recombinant ORF3a protein levels 48, 72 and 96 h post-transfection by Western blotting. For the detection of recombinant ORF3a, a primary polyclonal anti-His tag antibody was used. ORF3a protein expression normalized to GAPDH protein levels. ( c ) Detection of Green Fluorescent Protein (GFP) protein levels 48, 72 and 96 h post-transfection by Western blotting. For the detection of GFP, a primary polyclonal anti-GFP antibody was used. GFP protein expression normalized to GAPDH protein levels. The notation (***) indicate statistically significant differences ( p ≤ 0.001) compared to control cells.
    Figure Legend Snippet: Transient transfection of A549 cells with ORF3a-mRNA. ( a ) Evaluation of ORF3a mRNA levels at 48, 72 and 96 h post-transfection by real-time PCR. ORF3a mRNA levels were normalized to GAPDH gene. ( b ) Detection of recombinant ORF3a protein levels 48, 72 and 96 h post-transfection by Western blotting. For the detection of recombinant ORF3a, a primary polyclonal anti-His tag antibody was used. ORF3a protein expression normalized to GAPDH protein levels. ( c ) Detection of Green Fluorescent Protein (GFP) protein levels 48, 72 and 96 h post-transfection by Western blotting. For the detection of GFP, a primary polyclonal anti-GFP antibody was used. GFP protein expression normalized to GAPDH protein levels. The notation (***) indicate statistically significant differences ( p ≤ 0.001) compared to control cells.

    Techniques Used: Transfection, Real-time Polymerase Chain Reaction, Recombinant, Western Blot, Expressing, Control



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    Image Search Results


    Transient transfection of A549 cells with ORF3a-mRNA. ( a ) Evaluation of ORF3a mRNA levels at 48, 72 and 96 h post-transfection by real-time PCR. ORF3a mRNA levels were normalized to GAPDH gene. ( b ) Detection of recombinant ORF3a protein levels 48, 72 and 96 h post-transfection by Western blotting. For the detection of recombinant ORF3a, a primary polyclonal anti-His tag antibody was used. ORF3a protein expression normalized to GAPDH protein levels. ( c ) Detection of Green Fluorescent Protein (GFP) protein levels 48, 72 and 96 h post-transfection by Western blotting. For the detection of GFP, a primary polyclonal anti-GFP antibody was used. GFP protein expression normalized to GAPDH protein levels. The notation (***) indicate statistically significant differences ( p ≤ 0.001) compared to control cells.

    Journal: Biomedicines

    Article Title: Inhibition of PERK Kinase, an Orchestrator of the Unfolded Protein Response (UPR), Significantly Reduces Apoptosis and Inflammation of Lung Epithelial Cells Triggered by SARS-CoV-2 ORF3a Protein

    doi: 10.3390/biomedicines11061585

    Figure Lengend Snippet: Transient transfection of A549 cells with ORF3a-mRNA. ( a ) Evaluation of ORF3a mRNA levels at 48, 72 and 96 h post-transfection by real-time PCR. ORF3a mRNA levels were normalized to GAPDH gene. ( b ) Detection of recombinant ORF3a protein levels 48, 72 and 96 h post-transfection by Western blotting. For the detection of recombinant ORF3a, a primary polyclonal anti-His tag antibody was used. ORF3a protein expression normalized to GAPDH protein levels. ( c ) Detection of Green Fluorescent Protein (GFP) protein levels 48, 72 and 96 h post-transfection by Western blotting. For the detection of GFP, a primary polyclonal anti-GFP antibody was used. GFP protein expression normalized to GAPDH protein levels. The notation (***) indicate statistically significant differences ( p ≤ 0.001) compared to control cells.

    Article Snippet: PERK polyclonal rabbit primary antibody was obtained from OriGene (OriGene, Rockville, MD, USA).

    Techniques: Transfection, Real-time Polymerase Chain Reaction, Recombinant, Western Blot, Expressing, Control